The Effectiveness of Normal Saline Solution wtih Sodium Hypochlorite versus Hydrogen Peroxide as a Diabetic Foot Wound Cleanser

Iriss Ann F Ampang, Rodolfo M Cabrales, Amerah M Cabuntalan, Marinell E Corbita, Oswald M Lasaca, Mon Juleous C Medidas, Ariane B Omas-as, Rea Therese B Viduya


Diabetes mellitus is a chronic disease that can be severely complicated when the patient incur foot ulcer. In fact, development of vascular complications including failure of wound healing process has increased disease mortality over the years. There are different brands of antimicrobial cleansing agents dispensed in Davao City, many are expensive. The most common wound cleanser is Hydrogen Peroxide. This study aimed to perform a microbiological assay of Normal Saline Solution combined with Zonrox as a possible diabetic foot wound cleanser compared with Hydrogen Peroxide as positive control. The study utilized microbiological disk diffusion technique to determine the sensitivity of diabetic wound bacterial isolate by measuring the zones of inhibition (mm). The isolates were swabbed out of the wounds of three diabetic patients and cultured aseptically. Based on the reference sensitivity value of ≥16mm zone of inhibition, all bacterial isolates were are found to be sensitive to both the experimental and control agents. Interestingly, using ANOVA, there is a significant difference (p<0.05) in the zones of inhibition (mm) between Hydrogen Peroxide and Normal Saline Solution combined with Zonrox in favor for the experimental agent. Hence, the Normal Saline Solution combined with Zonrox exhibited greater zones of inhibition, and may be further tested as an alternative diabetic foot wound cleanser.

Full Text:



American Diabetes Study (position statement) Diabetes Care, 26 (1), 28-32. National Diabetes Information Clearinghouse, (2009) Information Way, Bethesda, MD 20892; 800-GETWELL or 301-6543327

Biomaster. (2009). Antimicrobials. Staffordshire Technology Park.

Centers for disease control and prevention (CDC). (2008). Diabtes Surveillance System. National diabetes fact sheet. Available at (accessed January 10, 2010).

Diabetes control and complication Research group Writing Team. (2002). Effects of intensive therapy on the microvascular complication of diabetes mellitus. Journal of the American Medical Association, 2563-2569.

Dufin, et al. (2006). High Plantar Pressure and Callus in Diabetic Adolescents. Journal of the American Podiatric Medical Association. Vol. 93 p. 214-220

Fajans, S.S., Floyd, J,C., Tattersall, R.B., Williamson, J.R., Pek, S., Taylor, C.I. The various faces of diabtes in the young; changing concepts Arch Intern Med 1976; 136; 194-202 Accesses January 11, 2010.

Green, H., De Ruiter, H-P., et al. (2002). Diabetes Expertise; A subspecialty on a general medical unit. MedSurg Nursing 281-287.

Guthrie, R.A. & Guthrie, D.W. (2004). Pathophysiology of Diabetes Mellitus. Critical Care nursing Quarterly, 113-125. Accessed January 10, 2010

Mogensen. (1997). Microalbuminuria, glycemic control, and blood pressure predicting outcome in diabetes type 1 and type 2.

Murray, Medical Microbiology, 5th Edition. (2005). World Health Organization. (2010). Diabetes Mellitus. Available at http;// November 28, 2009). Acceseed January 10, 2010.

Wild, S., Roglic, G., Green, A., et al. (2008). Global prevalence of diabetes: Estimates for the year 2006 and projections for 2030. P. 1047-1053


  • There are currently no refbacks.