Anti-angiogenic and Antioxidant Activity of Sayote (Sechium edule) Ethanolic Leaf Extract in Duck Embryo (Anas luzonica)

Kathleen G. Bersabal, Cherie G. Muaña, Jennalyn L. Dapitan, Jade Bianca S. Desabille, Apple Grace Charish B. Navasca, Rosecell Ann G. Ronquillo, Kristin Micah B. Sorongon


The study evaluated the anti-angiogenic and antioxidant properties of Sayote (Sechium edule) ethanolic leaf extract. Quantitative phytochemical analysis was done by estimating the total phenolic content by gallic acid in Sayote ethanolic leaf extract. To determine the anti-angiogenic property of the extract, Duck Chorioallantoic Membrane (CAM) Assay was employed. Results of the experiment showed that the ethanolic leaf extract has no median lethal concentration (LC50) since not more than 50% of the population of brine shrimp eggs died. It was found out that there is a significant difference between the antiangiogenic effects of the different concentrations of the leaf extract and that of the 95% ethanol as negative control in 11-day chick embryo chorioallantoic membrane. The formation of the branches of blood vessels is dependent on the treatment concentration: 5% concentration exhibited the least anti-angiogenic property while 15% showed the highest. In the antioxidant property, 2-2diphenyl-1-picrylhydrazyl hydrate (DPPH) assay was performed and result showed a significant difference between the antioxidant property leaf extract concentration and ascorbic acid (positive control). Results of the assay clearly indicated that the inhibition of free radical formation is directly proportional to
the concentration of extract. The 0.5% of Sayote exhibited the highest antioxidant property.

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De Vita, Helman S., Rosenberg S.A. (ed). Lippincott Williams and Wilkins, Philadelphia. pp509-519

Heeschen, C., Jang, J., Hoia-ky, V., Kaji, S., Yang, P., Hu, R.S. & Cooke, J.P. Nature Medicine:Nicotine is an agent of angiogenesis. Nicotine stimulates angiogenesis and promotes tumor growth and atherosclerosis. 7 (7):833-839

Henry, T.D., & Abraham, J.A. 2000. Review of preclinical and clinical results with vascular endothelial growth factors for therapeutic angiogenesis. Current Intervention and Cardiology Reports 2:228-241

Isner, J.A., & Asahara, T. 1999. Angiogenesis and vasculogenesis as Therapeutic strategies for postnatal neovascularization. Journal of Clinical Investigation 103:1231-1236

Lee, M.J., Yoon, S.H., Lee, S.K., Chung, M.H., Park, Y.I., Sung, C.K., Choi, J.S., & Kim, K.W. 1995. In vivo angiogenic activity of dichloromethane extracts of Aloe vera gel. Archives of Pharmaceutical Research 18:332-335.

Maciag, T., Mehlman, T., Friesel, R., & Schreiber, A.M. 1984. Heparin binds Endothelial cell growth factor, the principal cell mitogen in bovine brain. Science 225:932-935.

Plouet, J., Schilling, J., & Gospodarowicz, D. 1989. Isolation and characterization of a newly identified endothelial cell mitogen produce by AtT20 cells. EMBO Journal 8:3801-3807

Ribatti, D., Vacca, A., & Roncali, L. 1996. The chick embryo chorioallantoic membrane a model for in vivo research on angiogenesis. International Journal of Development Biology 40:1187-97

Shing, Y., Folkman, J., Sullivan, R., Butterfield, C., Murray, J., Klagsbrun, M. 1984. Heparin affinity:purification of a tumor derived capillary endothelial cell growth factor. Science 223, 1296-1298

Yancopoulos, G.D. 1986. Requisite role of Angiopoietin-1, a ligand for the TIE 2 receptor during embryonic angiogenesis. Cell 87:1171-118


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